FastQCFastQC Report
Thu 28 Apr 2016
ERR1041981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348739
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33410.14224654165490505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28320.12057533851143101No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23810.1013735455493352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.023.0955411
CGTATTA555.1452895E-420.18181815
AACGGTA801.6178414E-518.518
TCTTATA46400.016.7456937
GTATTAG3150.016.4444451
GTACCGA803.3844457E-416.18756
CTTATAC25700.015.98054437
CGCTATA1302.5921327E-715.6538462
TATACAC10900.015.44495437
GTATCAA35950.015.1293472
TAGTACT2850.014.9298244
TATACCG908.278547E-414.3888885
ATACCGA908.278547E-414.3888886
AGTCGTA800.00630120213.87512
CTAGTAC2051.4260877E-913.5365853
TTAACGA850.00940855313.0588234
TTACGGT1000.0018334712.9500014
CTCTTAT71400.012.79972137
TATTAGA3350.012.7014932
TTATAGT3100.012.5322574
Produced by FastQC (version 0.11.5)