FastQCFastQC Report
Thu 28 Apr 2016
ERR1041979.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041979.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6262949
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT205530.3281680882280855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173440.27693024484152756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144250.23032280799348678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68950.020.4184191
CTTATAC61800.017.99110237
TCTTATA112150.016.36379837
GTATCAA100850.013.8681212
CTCTTAT176150.013.65313637
TATACCG2202.8558134E-1013.4545455
TAGACGT3051.8189894E-1212.1311475
GTCTATA5200.012.0961541
GTGTAAG9950.011.8994971
ATCAACG145150.011.6238372
TATACAC25100.011.49800837
TATCAAC147250.011.4957561
CGTATAC1951.9641066E-611.3846163
TCAACGC149250.011.3541043
TAATACT11650.011.2746784
CAACGCA150350.011.234124
TTAGACG2801.0622898E-911.2321434
GTACTAG4500.011.11
CTAGTAC5850.011.0683763
AACGCAG157050.010.8255335
Produced by FastQC (version 0.11.5)