FastQCFastQC Report
Thu 28 Apr 2016
ERR1041978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3456791
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89570.25911314858202306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85010.24592172335556303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64120.18548995296504764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.021.90231
TATACCG1451.546141E-1017.8620685
CTTATAC31950.016.44444537
TCTTATA54500.016.02201837
GTATCAA51850.014.7000962
GTCTTAC1753.572677E-813.7428581
CTCTTAT85550.013.08299337
TACACCG2501.6007107E-1012.5800015
TATACAC12400.012.38306437
TTAGACT3002.3646862E-1111.7166674
GTATTAA4600.011.6630431
GTATTAG6550.011.58015251
GTACTAG2401.4882971E-811.5625011
GACCGTG4100.011.2804887
ATCAACG75200.011.2180852
TACACTG6950.011.1798565
TCTCTTA125300.011.14724537
CAACGCA77350.011.0497734
TCAACGC77350.011.0258563
TATCAAC76950.010.9870051
Produced by FastQC (version 0.11.5)