FastQCFastQC Report
Thu 28 Apr 2016
ERR1041977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6319422
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194190.3072907617183977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162720.2574919035316838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139120.22014671594965488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC66950.017.49141337
GGTATCA91900.016.1245921
TCTTATA110250.015.84036337
TATACCG2400.014.6458335
CTCTTAT174550.013.61930637
TAGTACG4200.012.7738094
TATACAC21450.011.98834437
ATACCGA2705.802576E-1011.6481486
GTATCAA128550.011.5562042
GTATTAG12800.011.4179691
CGCGTAT1150.005222876611.2608712
AATACTG16300.011.2361975
GTCTAGA5700.011.0350881
TAATACT16100.011.03105454
CTCTAAT10750.010.8418611
CGCTATA2401.8464198E-710.7916662
CTACACT10650.010.7699544
GTACTAG3657.2759576E-1210.6438351
GTAGCAC5050.010.6237633
CGAACTA5650.010.47787729
Produced by FastQC (version 0.11.5)