FastQCFastQC Report
Thu 28 Apr 2016
ERR1041976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891698
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67320.3558707573830495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28440.15034112210299955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25190.13316078993581426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC358.871521E-426.4285724
CTAACGC400.001931645823.1253
CTTATAC24600.016.84552837
GTCTAAC904.4483997E-516.4444451
ATTAGAG3100.016.1129043
CGATTAA1508.109237E-814.822
GTATTAG6500.014.5153861
CGTTTCG908.2774984E-414.38888823
TATACAC8050.014.01863437
TTAACGG2405.456968E-1213.87499935
TATTAGA4700.013.7765962
TTACACT2601.8189894E-1213.5192314
GTACTAG1451.3729701E-512.758621
TAGCGGA1602.6974176E-612.7187517
ACCGTGT1602.6974176E-612.718758
GTATAGG3950.012.645571
AACGCAG41500.012.5710835
TATACTG2653.274181E-1112.5660385
GTATTGG3001.8189894E-1212.3333331
CCTACAC3300.012.3333333
Produced by FastQC (version 0.11.5)