FastQCFastQC Report
Thu 28 Apr 2016
ERR1041975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478222
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110540.3178060514826253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49080.14110657686599648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45610.13113021538015687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC34900.016.96275137
TAGACCG700.002593910315.8571425
TAATACT10150.015.492614
GTATTAG8800.015.1363641
GGTATCA30250.015.1057851
TCTAATA7600.014.8486842
TATACTG6450.014.6279075
AACGCAG61900.014.525045
TAATACG2059.276846E-1114.4390254
TCAACGC62750.014.3282873
TTAGCGA1551.2123019E-714.3225827
ATCAACG63200.014.1677232
CAACGCA63650.014.0675564
CTAATAC8950.013.8491613
TAGACAG4650.013.5268835
TAGAACA4650.013.5268834
CTCTAAT5900.013.483051
ATAATAC5550.013.3333333
ACGCAGA68000.013.1404426
TATCAAC69000.013.0304351
Produced by FastQC (version 0.11.5)