FastQCFastQC Report
Thu 28 Apr 2016
ERR1041974.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041974.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245423
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.1708663659663595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC54800.017.95985437
TATACAC17450.014.7363937
GGTATCA34900.014.6303731
TCTTATA89000.014.50898837
TATACTG6000.014.4916675
TTAACGG3450.014.47826135
GTATTAG10250.014.4390241
AACGCAG61200.014.3888885
ATAATAC5850.013.9145293
TAGACAG5750.013.8347835
TCAACGC64950.013.5581223
CAACGCA65150.013.51654
ATCAACG65250.013.439082
TAATACT9850.013.1472074
ACGCAGA68100.012.9853166
TAACGGC3850.012.97402736
TAAGCCG2305.7661964E-1012.8695655
TTTAACG5050.012.45544634
AGAGTAC70450.012.34208710
TATTAGA9150.012.3333342
Produced by FastQC (version 0.11.5)