FastQCFastQC Report
Thu 28 Apr 2016
ERR1041973.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041973.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4554944
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135950.2984668966292451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61110.1341619128577651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52660.11561064197496171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC59000.017.08898437
CGCGAAA1101.4536681E-515.13636415
GTATTAG13350.014.8277161
TCTATAC4800.014.2604173
TAATACT13300.014.187974
AGTACCG6200.014.0241945
CGAACTA6650.013.63157924
GTATAGA6000.013.5666661
AACGCAG105250.013.0950125
TCTTATA87100.013.02009237
TATACAC19150.012.94516937
GTATTAA5100.012.6960781
TCTAATA11500.012.5478262
GGTATCA64400.012.5248451
GGTAAAC7250.012.503448535
TACGCAG1359.972589E-512.3333335
GGACCGT2552.237357E-1012.3333336
CTCTAAT9000.012.3333331
ATACCTT8850.012.3333336
CTAATAC12850.012.2373543
Produced by FastQC (version 0.11.5)