FastQCFastQC Report
Thu 28 Apr 2016
ERR1041972.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041972.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5538621
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164780.29751087861039777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78880.142418121767133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65960.11909101561561986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC72500.017.4793137
TATACAC24550.014.317718537
AACGCAG123400.013.9124795
GGTATCA70750.013.6233221
TCTTATA108750.013.47310437
GTACTAG3600.013.3611111
TACCCCG8100.013.2469145
CGAACTA8800.013.03409124
AATTTCG8050.012.86956528
CAACGCA135100.012.7487044
ATCAACG134300.012.7144452
ACGCAGA135100.012.70762356
TCAACGC134550.012.6908223
ATTAGAG12550.012.6772913
GTATTAG17800.012.5758421
TATACTG6650.012.5187975
TATTAGA14200.012.5070432
TTATACA37300.012.39946537
CGCAGAG139200.012.2801737
GCGAACT9350.012.26737923
Produced by FastQC (version 0.11.5)