FastQCFastQC Report
Thu 28 Apr 2016
ERR1041971.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041971.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843524
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74430.19365041040461825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC39850.017.08406437
TATACTG6900.016.3550725
GTATTAG10750.016.004651
TAATACT13000.015.9384614
TAATACG2700.015.7592594
CTCTAAT8750.015.6457151
CTAATAC12700.015.4409453
TTAACGG4550.015.04395735
TCTATAC4600.014.8804343
AATACTG12550.014.5936255
GTCTATA2950.014.4237291
TCTAATA11050.014.398192
TTAGCGA2950.013.7966127
ATAATAC7000.013.7428583
ATACTGG12800.013.730476
CTACACT7050.013.64539054
AATACGC3200.013.2968765
TACCCCG3800.013.1447375
CCTATAC9150.013.1420773
AACGCAG58250.013.1167385
Produced by FastQC (version 0.11.5)