FastQCFastQC Report
Thu 28 Apr 2016
ERR1041970.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041970.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3570081
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.11097787417148237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC41200.019.2633537
CGACTAA1653.6379788E-1217.93939437
TACGTCG1054.80426E-717.61904721
TCGCTAA2250.015.62222214
TAAGACA5700.014.6052644
GTATTAG11300.014.5707971
TTAGACT3850.014.4155844
TAATACT10300.014.3689334
CTCGCTA2600.014.23076913
CTAATAC10400.014.2307693
ATTAGAG7100.014.0704223
TATTAGA9350.013.8502662
TCTTATA63650.013.83503437
GGTATCA25250.013.7742581
TTAACGG5150.013.65048635
CGTATAC1501.3075896E-613.5666673
AGTTCGC4500.013.5666678
CTAAACT9000.013.3611124
TAGTACG3600.013.3611114
GTCTATA2501.0913936E-1113.321
Produced by FastQC (version 0.11.5)