FastQCFastQC Report
Thu 28 Apr 2016
ERR1041969.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041969.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072726
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.16770358880086805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA250.005496181529.613
CTTATAC10950.019.59817337
TCTATAC1750.019.028573
TTAGACG500.007034582618.54
TCGTATG500.007034582618.510
GTATAGA1002.8744762E-718.51
AATTTCG1156.403752E-817.69565228
GTCGGTT1054.7965295E-717.61904712
TAACGAG650.001579840817.0769235
GTCCTAA2250.016.4444451
TGGACCG803.3823022E-416.18755
ACAGCGT1059.341855E-615.8571428
CGAACTA1302.5882946E-715.65384624
GCGAACT1302.5882946E-715.65384623
CCTATAC2150.015.4883723
TAGACAG1201.93511E-615.4166665
CTATACA1455.3434633E-815.3103454
GGGTAAG1455.3434633E-815.3103451
TTTAGAC1353.9711267E-715.0740753
ATCTATA1353.9711267E-715.0740752
Produced by FastQC (version 0.11.5)