FastQCFastQC Report
Thu 28 Apr 2016
ERR1041968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400479
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.19728964161547588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA450.003826219820.5555551
TTAATAC1750.020.0857143
CTTATAC16550.016.54380637
TTAAGAC1151.2425935E-616.0869563
GTTCTAG2200.015.9772731
TATAGAC700.002592872815.8571423
GTTTACC2500.015.54000115
TATAGAT1557.210474E-915.51612955
TAGACAG2450.015.102045
TTATGCG1252.9596777E-614.8000014
TTAGCGA1001.09363435E-414.827
TAGGTAC2251.8189894E-1214.83
TAGCGAG1001.09363435E-414.828
ATCTTAC2750.014.81
TCGTTAC750.00410500214.79999913
CGTGTTG750.00410500214.79999910
ATTAGAG2301.8189894E-1214.4782613
TAGACTC1152.2104154E-514.4782615
CTTACCC2950.014.4237293
ACTGTTT4750.014.0210528
Produced by FastQC (version 0.11.5)