FastQCFastQC Report
Thu 28 Apr 2016
ERR1041967.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041967.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307970
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76390.23092712449024627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34170.10329597910501001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC38700.017.63953637
TTTAGCG1554.0199666E-1016.70967726
TATACCG1452.9849616E-916.5862065
CTAACGC803.3849684E-416.18753
CGCGAAA700.002593873315.85714315
TCTATAC3150.015.8571423
TATACTG4150.015.1566275
CTCTAAT6550.014.1221381
CTAATAC10250.014.0780493
TACCCCG4350.014.0344835
TTATGCG3450.013.9420294
TCTTATA58550.013.93424437
TTGCGAT800.006301919513.87516
TACGGTC1203.3040436E-513.87499910
ATTAGAC2551.8189894E-1213.7843133
CGAGTTC4450.013.71910214
CCGAGTT4600.013.67391213
ATACCTT5850.013.5982916
TCTAATA7400.013.52
CGAACTA4600.013.27173824
Produced by FastQC (version 0.11.5)