FastQCFastQC Report
Thu 28 Apr 2016
ERR1041966.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041966.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147253
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT244430.7766455381883821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT242710.7711804548283853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182300.5792352886787303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC170020.5402171353875904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG123350.3919290886369796No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA109280.34722343580258724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108300.3441096092370076No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG72780.23124928310498077No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG69470.22073217501103345No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC61220.19451883912732787No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG57530.18279432889570682No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT56520.17958518110873198No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA47580.15117945713293465No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA47240.15009914995712134No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG40500.12868364888364553No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40190.1276986629292275No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC38430.12210648460737031No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38150.12121681987434757No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35930.11416304949109587No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC32010.1017077432287776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1750.020.0857143
AAGACGG9500.018.3052645
CAAGACG11550.016.1774884
CGATTAG1151.2437977E-616.08695616
GACGGAC10950.016.0502287
ACGGACC10850.016.0276498
CGGACCA11000.015.9772729
AGACGGA11100.015.8333336
CGCAAGA11300.015.5530982
CGAACGA3700.015.016
CGGTCCA5700.014.92982410
TAGTACT3350.014.9104484
GTATTAG9100.014.8406591
ATCACGC4750.014.4105263
GCGCAAG12100.014.37190151
GCAAGAC14200.014.3309863
ACGAACG3950.014.05063415
CGAGCCG10300.014.00970915
TATCACG4800.013.8749992
TATAACG3750.013.8133342
Produced by FastQC (version 0.11.5)