FastQCFastQC Report
Thu 28 Apr 2016
ERR1041964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419767
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47921.1415856892037726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47761.1377740508424912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.5355351897600336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22000.5241002746761895No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.15175085225851484No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.13126329606662743No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.11935192618762314No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.11625497001908201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT250.00549241829.64
CCGCCTA250.00549241829.615
GTTATGC250.00549241829.63
CGTAGCT454.0002906E-628.77777925
GCGTAGC454.0002906E-628.77777924
CGCCTAT358.859978E-426.42857234
ACGCTCC358.859978E-426.4285726
TTGCGTA509.073146E-625.90000222
TACTGAG400.001929156523.1252
TCTAGTG502.698144E-422.228
CTACCCG502.698144E-422.210
CGATGTC502.698144E-422.223
TATACTG502.698144E-422.25
GTACGAT502.698144E-422.220
TATTAAC603.7190373E-521.5833322
GTATACT603.7190373E-521.5833324
ACGGACC1059.767973E-1021.1428578
GGCGTTA450.003821822820.55555731
GACCAGA909.442374E-820.55555711
CGTGTCG450.003821822820.5555579
Produced by FastQC (version 0.11.5)