FastQCFastQC Report
Thu 28 Apr 2016
ERR1041963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21966949
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT993920.4524615594090922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT893350.40667914328931154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT766970.3491472575458704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445870.20297311201478183No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC275600.1254612099295173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC17050.017.90322736
TATAACG15950.017.862072
TTAACGG17100.017.52631635
AAGACGG53050.016.5994345
ACGGACC56150.015.28762158
TCTAGCG35000.015.27571528
GACGGAC55850.015.137877
CTAGCGG36350.014.8101829
TAACGCA20450.014.38386254
CGCAAGA59900.014.3614352
GTATTAG82900.014.2376361
CGGACCA61250.013.9240829
TAATACT72900.013.8559674
AATCGTC14050.013.82562328
TAAACGC49100.013.79022428
ATTAGAG48900.013.6952963
GTATAAC29100.013.66838551
AGACGGA62500.013.64566
CGGTCCA41550.013.62454910
TATACTG30850.013.6126415
Produced by FastQC (version 0.11.5)