FastQCFastQC Report
Thu 28 Apr 2016
ERR1041961.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041961.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14312144
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT637220.4452302883481329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT522370.36498375086220486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT473830.3310684967954487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277400.1938214148767648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG13050.017.72030635
TAACGGC13100.017.65267236
AAGACGG35400.016.0437855
ACGGACC36200.015.4848068
GACGGAC35350.015.3861387
CGCAAGA36150.015.0968192
TCTAGCG23050.014.68763528
CTAGCGG23250.014.6408629
CGGACCA39050.014.49679859
TCTATAC21950.014.41233
AATACTG34250.014.3678835
ATTAGAG32450.014.3667183
CGTATTA17100.014.17251515
TCGGTAA5500.014.12727227
AGACGGA39500.014.0037976
TATACTG20300.013.943355
TAATACT54500.013.8834874
TTAGACT17100.013.8479534
TATAACG7850.013.668792
CGAACGT12400.013.5766134
Produced by FastQC (version 0.11.5)