FastQCFastQC Report
Thu 28 Apr 2016
ERR1041960.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041960.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005791
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT453730.4534673970303797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT377910.3776912789803425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT337240.3370448173462748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201260.2011435177888485No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC123860.12378831418725417No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT103410.1033501499281766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG25600.016.9101565
GACGGAC26100.016.0191577
ACGGACC26850.015.6405958
CGCAAGA27700.015.4945842
TATAACG5200.015.2980772
TCTATAC13900.014.6402873
AGACGGA29000.014.54482656
TCTAGCG18500.014.328
TAATACT30500.014.1934424
CGGACCA29750.014.05378159
CTAGCGG19350.013.76744129
CCGTTTA4050.013.70370413
TTAACGG6700.013.52985135
GTATTAG36550.013.5143631
TATACTG15250.013.4655745
TAACGGC6900.013.40579736
ATAACGC23350.013.3897213
CAAGACG33350.013.3133434
GCGCAAG33450.013.1076231
TAACGCC15850.013.07255654
Produced by FastQC (version 0.11.5)