FastQCFastQC Report
Thu 28 Apr 2016
ERR1041959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18117694
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT678460.37447370509734845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT558090.3080358902187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT512570.28291127998960575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285900.1578015392025056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG43850.016.2850635
TTAACGG11200.016.02232235
ACGGACC43500.015.9057478
GACGGAC43200.015.5879637
CGCAAGA47750.014.8387432
CGTATTA18750.014.79999915
TTTAGCG16700.014.73353326
TAACGGC12300.014.58943136
CGGACCA49100.014.3930759
TCTAGCG28700.014.37456328
GTATTAG68700.014.3529831
AGACGGA48400.014.1807856
TACGTAG16150.014.08978414
CTAGCGG29150.014.08919329
TCTATAC26700.013.9269663
TATAACG9300.013.9247312
GTACGTA16100.013.90372713
AATCGTC14600.013.68493228
CCGTATT19650.013.55725114
TATACTG27700.013.4241885
Produced by FastQC (version 0.11.5)