FastQCFastQC Report
Thu 28 Apr 2016
ERR1041958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8474993
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT789870.9320007697941461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT662790.7820537432892275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT517540.6106671710525307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337510.39824221683722927No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC117380.13850158932284662No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100150.11817118904994967No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95150.11227147916228367No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT91010.10738651937529625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG21050.016.2589075
ACGGACC20550.015.6642338
CAAGACG24400.014.8606554
GACGGAC21550.014.6798157
CGCAAGA23100.014.4956712
TAGACCG2850.014.2807035
CGAACGA12550.014.15139516
CGGACCA22750.014.149459
AGACGGA23750.014.0210526
ATAACGA13450.013.75464712
TATACTG15400.013.6948065
TTAACGG3800.013.63157935
TAACGAA13300.013.4924813
CGAACGT5650.013.4247784
TAATACT19450.013.1259644
ACGAACG13750.013.0509115
GCGCAAG26100.012.9003831
TCGAACG5750.012.8695653
TAACGGC4350.012.75862136
GCAAGAC36350.012.5708393
Produced by FastQC (version 0.11.5)