FastQCFastQC Report
Thu 28 Apr 2016
ERR1041957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16335602
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1217550.7453352499650763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1133190.6936934433147918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT894160.5473688695402839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT545300.33381077722143326No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT203660.12467247916544491No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177710.10878693053368955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG47650.018.4029395
ACGGACC48500.017.622688
GACGGAC48250.017.5989637
TCTAGCG28500.016.94210428
CTAGCGG29700.016.38215429
CGCAAGA53000.016.3707542
AGACGGA53550.016.0298796
CGGACCA54250.015.8230419
CAAGACG58500.015.8119664
GCGCAAG59450.014.6879731
GCAAGAC70800.014.5805083
TAAACGC34550.014.13603428
CGGTCCA35650.014.11500710
ACGCTTC35900.014.01671331
CGAACGA23300.013.81545116
TCTATAC20850.013.7529973
TAGCGGC36550.013.716825530
GTAAACG35600.013.66713427
TTAACGG7600.013.63157935
CGACGGT38750.013.3677437
Produced by FastQC (version 0.11.5)