FastQCFastQC Report
Thu 28 Apr 2016
ERR1041956.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041956.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5977511
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347520.5813791057849997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261820.4380083951330245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217210.3633786704867628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121210.20277670756272972No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT82490.13800058251670302No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC65180.1090420410769633No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT61800.1033875136323463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18350.018.5504075
TCTAGCG10800.018.32870528
GACGGAC18750.017.3653327
CTAGCGG11500.017.21304329
CGGTCTA650.001580983717.07692320
ACGGACC19250.017.010398
CGCAAGA19700.016.9974632
TACCGTC12900.016.779077
CAAGACG21950.016.5193614
ACCGTCG12950.016.2857158
ATACCGT14450.016.003466
TTCGGGC18600.015.71505435
TATACTG9000.015.6222225
TAAACGC18800.015.54787328
CGGACCA21100.015.5189569
CGTCGTA13550.015.42804410
CGAACGA8100.015.30246916
ACGCTTC19000.015.28684231
GCGCAAG22300.015.1816151
GTAAACG18950.015.13192727
Produced by FastQC (version 0.11.5)