FastQCFastQC Report
Thu 28 Apr 2016
ERR1041955.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041955.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734770
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT499000.8701307986196483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT402620.7020682608020897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT331590.5782097625536856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194630.3393858864435714No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT67350.11744150157722107No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62730.10938538075633372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG14850.018.9360285
GACGGAC15000.017.8833357
ACGGACC15150.017.8283828
AGACGGA16300.017.2515346
TCTAGCG9500.016.94210428
CGCAAGA16600.016.8283142
CTAGCGG9850.016.52791829
CGGACCA17200.016.0261639
GCGCAAG19300.014.9533681
CAAGACG20550.014.8540144
TTAACGG2600.014.23076935
TAGCGGC12350.013.48178130
GAGCGAA20900.013.36602916
GCGAAAG20350.013.36363618
TATACAG10250.013.175615
GTCCTAT14350.013.1498261
CGGTCCA12300.013.08536510
GCGTTAG850.00941071213.0588241
TAACGCC7950.013.0314464
CGAAAGC20600.013.02184519
Produced by FastQC (version 0.11.5)