FastQCFastQC Report
Thu 28 Apr 2016
ERR1041954.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041954.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444315
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT527031.185852037940605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT444701.0006041425956531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT360210.8104961056990785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179890.40476428875991016No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCT54510.1226510722124782No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.1216160420672252No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53390.1201309988153405No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGA52220.11749842214154486No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCG48940.1101182071927845No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTT48510.10915067901352626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG46910.10555057416047242No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTT45990.10348051386996646No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45630.10267049027802935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45360.10206297258407653No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA45060.10138795292412892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC7900.018.7341773
CTAACGC1306.9849193E-1018.53
CGTAGCG5000.018.515
GTACTAG2150.018.0697671
TTAACGG4550.017.48351735
CTTACCG852.7259606E-517.4117649
TCGTAGC5350.017.28971914
ACGGACC3000.017.2666668
CGAACGT2050.017.1463434
ATTAGAG7050.017.0567363
TCTAACG1554.0199666E-1016.7096772
CCCAACG2250.016.44444521
GCGGAAT5900.016.30508419
GGCACCG6200.016.1129029
CTCTATA7700.016.0974032
GACGGAC3150.015.8571427
TCGAACG2100.015.8571423
GGTATCA225750.015.7915841
TATTAAC14450.015.7474052
CTACCCG3350.015.462687510
Produced by FastQC (version 0.11.5)