FastQCFastQC Report
Thu 28 Apr 2016
ERR1041953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8285131
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT278850.3365667965901807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT232970.2811904844956586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176630.2131891457117576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95650.11544778229819178No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87420.10551432439631914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG12150.016.59670828
GACGGAC19550.015.8030697
AAGACGG21250.015.75764755
ACGGACC20050.015.5935178
CTAGCGG12900.015.48837129
CGAACGA13150.014.91254816
TACTCCG6700.014.9104495
CGGACCA21900.014.6986299
CGCAATA14600.014.19178136
CGCAAGA21650.014.1847582
TACCGTC15950.014.0344827
AGACGGA22850.013.9256016
TATACTG12650.013.7470365
ATAACGA14600.013.68493212
TAACGCC14650.013.6382254
TACGACG17100.013.5233925
GCGCAAG23800.013.4474781
TCGTTTA13350.013.44194830
ATAATAC16150.013.4024773
GGTATCA171300.013.3161121
Produced by FastQC (version 0.11.5)