FastQCFastQC Report
Thu 28 Apr 2016
ERR1041952.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041952.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260280
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT551531.2945862713248895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT469401.1018055151304609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT387600.9097993559108791No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188870.4433276686039415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC72000.16900297633019426No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA60510.14203291802416743No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC56720.1331367891312308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT54670.12832489883294057No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG53520.12562554573877774No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52610.1234895358990489No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.11407700902288113No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG45910.10776287004610026No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA45300.1063310392744139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT500.00703793218.535
CGGTCCA2300.018.510
GTACCGT752.0689217E-417.2666666
CGGTAAG904.4505043E-516.44444528
CGTAGCG1708.54925E-1116.3235315
TTAACGG2400.016.187535
TGCGACG1059.354897E-615.85714222
CGGTCTA1902.7284841E-1115.57894820
ACGGACC4450.015.3820228
TAGACGA855.368383E-415.2352945
CCGTTAG855.368383E-415.2352949
CTCTATA3550.015.1126772
CCGCCTA2600.014.94230715
GCGGAAT1904.5656634E-1014.60526419
CCGGTCC3250.014.2307699
AAGACGG6000.014.1833335
CGCAAGA4850.014.1134022
CGGACCA4850.014.1134029
TCTATAC5000.014.0599993
GTCGGGA2252.7284841E-1113.97777752
Produced by FastQC (version 0.11.5)