FastQCFastQC Report
Thu 28 Apr 2016
ERR1041951.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041951.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970641
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18600.19162594615310913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.14227711378357188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13060.1345502611161078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.025.7118661
TGCGTAC400.001930971923.12512
ATTAGAC450.003825385620.5555553
GTTCGTA555.1423034E-420.18181825
CGCTATT500.007034112718.526
GTATCAA8950.017.1564252
TGTATAC1107.8045196E-716.8181823
TAATACG700.00259210415.8571434
CGTGCGT700.00259210415.85714310
TAGGCTG2450.015.1020415
TATACTG1601.0953954E-815.031255
CCTATAC1601.0953954E-815.031253
GTCTAAG1001.0930853E-414.81
CCGCCGT1508.0952304E-814.79999924
CCTGTAA8550.014.4970761
TACACTC1152.2090337E-514.4782615
TCTGTCG1304.4419594E-614.2307688
TGTAATC7300.014.1917813
GTGTAGA3000.014.1833321
TAGATCA1051.6552124E-414.0952384
Produced by FastQC (version 0.11.5)