FastQCFastQC Report
Thu 28 Apr 2016
ERR1041950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996068
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111380.37175391212749515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95240.31788330571936285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78330.2614426641851921No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA51090.17052349946663428No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC47770.15944230905306556No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA38460.12836824798369062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.1254978191416216No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG34300.11448338288717079No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC33840.11294803722745946No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG32110.10717380246376251No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC31260.10433675070125244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.018.5319521
AAGACGG6850.018.094895
ACGGACC6550.017.511458
TCTAGCG3750.016.77333328
TCGTTTA4850.016.40206130
CGCAAGA7900.016.158232
TCTATAC3250.015.9384613
TTAACGG1751.3278623E-1015.85714335
CGCAATA4350.015.73563336
CGAACGC1302.592824E-715.65384630
TATAGCG957.065729E-515.5789482
CGTATTA1601.0984877E-815.03125115
CGGACCA7600.014.8486849
AGACGGA7750.014.8000016
CGTTTAT5500.014.831
CTAGCGG4250.014.829
TGGTCGG5400.014.38888937
GCGCAAG8900.014.3426961
TACGACG4300.014.1976745
GACGGAC7950.014.1949687
Produced by FastQC (version 0.11.5)