FastQCFastQC Report
Thu 28 Apr 2016
ERR1041949.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041949.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7892947
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT600320.7605777664540254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT486190.6159803176177415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC384880.48762521780521273No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG376920.4775402647452213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT371880.47115481707909607No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA235980.2989757817960769No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG215160.27259780155625013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197920.2507555162856155No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT124820.15814118604875974No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG120130.15219917224833765No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG117120.14838564100329066No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC108780.13781924546053584No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC93060.11790273012095483No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88150.11168198646209077No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA81390.10311737808451013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG6600.016.537885
GTATTAG25100.015.5517921
TTTAGCG5950.015.54621926
GACGGAC14100.015.4822697
ACGGACC14300.015.3951058
AAGACGG15100.015.314575
CGTATTA6100.015.16393515
TCTATAC10300.014.9077673
TCGCTAA3600.014.90277914
TTAGCGA4600.014.88043427
TAATACT24200.014.8305784
TTAACGG8800.014.71590935
TCTAGCG9750.014.61025628
TAACGGC9000.014.59444436
TAACGCC14500.014.54482654
TCGTTTA12700.014.4212630
TTAGACT7650.014.2679744
ATTAGAC6950.014.1079143
TAAACGC13700.013.90875928
CGCATCG13750.013.85818113
Produced by FastQC (version 0.11.5)