FastQCFastQC Report
Thu 28 Apr 2016
ERR1041948.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041948.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12389209
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT333600.26926658513872836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT313660.25317193373685115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT245040.19778502404794365No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT134480.10854607424896939No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126620.10220184355595262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG33150.017.0211165
ACGGACC32000.016.7078138
TCTAGCG20950.016.6014328
CTAGCGG21300.016.41549329
GACGGAC32550.016.3118277
TCGTTTA22650.016.17218630
GTATTAG38750.015.1341931
CGCAAGA37350.015.0575632
TTAACGG8000.015.03125135
CGGACCA36250.014.8510369
AGACGGA36650.014.8403816
TAAACGC27650.014.71971128
GTAAACG27800.014.57374127
CAAGACG41350.014.3168084
CGAACGA15600.014.23076816
TAACGGC8350.014.17964136
ACCGTCG23500.014.0127668
CGTATAC3450.013.9420293
CGTTTAT26700.013.78838931
TACCGTC24650.013.734287
Produced by FastQC (version 0.11.5)