FastQCFastQC Report
Thu 28 Apr 2016
ERR1041947.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041947.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248764
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT404210.9513590305321735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT395680.931282603599541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT267790.630277417150023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154440.36349394788696193No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA49010.11535119390015543No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46010.1082903169015742No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC44290.10424208075572096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC8450.019.0473388
GACGGAC8650.018.3930647
AAGACGG9200.018.2989145
AGACGGA9500.017.1368436
CGGACCA9700.016.5927839
TTAACGG3750.016.2835
CGCAAGA10350.016.2657012
AATACTG10600.015.3584915
ATTAGAG7950.015.358493
TAACCGG2550.014.50980422
GTATTAG14750.014.4237291
TATACTG4550.014.2307695
TAACGCC7300.014.1917814
CACGCGA3000.014.18333324
GGTCTAG3800.014.1184221
TAACGGC4200.014.09523836
CTAGTAC2101.364242E-1014.0952383
CTAGCGG5400.014.04629529
TAATACT14200.013.9401414
CCGTCGA1203.3044773E-513.8750019
Produced by FastQC (version 0.11.5)