FastQCFastQC Report
Thu 28 Apr 2016
ERR1041946.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041946.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396157
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27910.19990588451012314No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG26750.1915973633337798No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC19380.13880960379097765No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA16970.12154793479529881No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16570.11868292749311145No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15460.1107325322295415No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG14730.10550389390304958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC450.003826214220.5555553
CTTACAC1254.129106E-1019.243
TATACTG1950.018.974365
TAACGAA1800.018.513
TATTAGG1054.7990943E-717.6190472
TTAGGCC953.607509E-617.5263164
GTCCTAA1502.5102054E-1017.2666661
GTTCTAG1301.3937097E-817.0769231
GTATTAT1107.8105586E-716.8181821
GACTGTA1655.4569682E-1116.8181827
CGTTTAC1107.8105586E-716.81818233
AGACGGA2800.016.5178596
AGTACCG904.447058E-516.4444455
CCGATAA2050.016.2439029
ATAACGA2050.016.24390212
ACGAACG2050.016.24390215
GTTCAAA3900.016.1282061
TTAGATA700.002592867215.8571434
CCTTTAT1403.4744517E-815.8571431
ATTTCGG700.002592867215.85714329
Produced by FastQC (version 0.11.5)