FastQCFastQC Report
Thu 28 Apr 2016
ERR1041945.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041945.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784952
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC129420.725061514259207No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG106610.5972709630286976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97280.5450006498774197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79550.4456702477153447No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA79380.44471784115203095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58710.3289164078361771No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG58220.326171235977214No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC48010.2689708182629001No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38950.21821315082982623No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT37360.20930534826706826No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG36030.2018541675070254No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA31660.17737171643831318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31380.17580304680461994No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30100.16863198562202233No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG29960.16784765080517572No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT27720.15529829373562987No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC25260.14151641052532504No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24390.13664232987777825No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC18780.10521291328842457No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC18270.10235569359848332No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG17970.100674976133812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA450.003826624220.5555571
AAGACGG3650.020.2739725
ACGGACC3650.019.7671228
TCTAGCG2500.019.2428
CGCAAGA3750.018.7466662
CTAGCGG2600.018.529
ACGTTAG500.007036370718.4999981
TTAGACT1351.1532393E-917.8148164
TATACTG2600.017.7884625
GACGGAC3900.017.5512837
CGGACCA4350.017.4367839
CAAGACG4600.017.2934784
GAACCGT752.0680735E-417.2666666
TTAACGG1107.813542E-716.81818435
CGATCGG1107.813542E-716.81818436
GCGCAAG4800.016.18751
GTATTAG5150.016.1650491
TAGACAG2100.015.8571425
TAGGTCG700.002593247215.85714221
GCGTCGC700.002593247215.85714211
Produced by FastQC (version 0.11.5)