FastQCFastQC Report
Thu 28 Apr 2016
ERR1041944.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041944.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3251495
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC161900.49792480074550327No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG127360.39169674257533843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125750.38674517414297116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107080.3293254333775694No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA98980.3044138157985788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76390.23493808232828284No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG70060.21547011451655318No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC55680.17124430454298717No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48570.149377440223651No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT45630.14033544569498033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45030.13849014068912915No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG43650.1342459391756715No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT40890.12575753614875618No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA37710.11597741961774508No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG35880.11034923934989906No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC35120.10801185300915424No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT34930.10742750642396806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG9500.017.7210525
CGCAAGA10300.016.8834932
GTAAACG7900.016.8607627
TCTAGCG6150.016.8455328
TAAACGC7950.016.75471728
ACGGACC10150.016.5862068
GACGGAC10150.016.5862067
CTAGCGG6150.016.54471629
ACGCTTC8400.016.29761931
TTCGGGC8150.016.11656635
CGGACCA10350.016.0869569
CGCTTCG8800.015.97727332
AGACGGA10800.015.7592596
CGAACGA5000.015.5416
AAACGCT8750.015.43428429
ACTCTAA9150.015.36612110
CACTCTA9400.015.3510639
ACGAACG5100.015.23529415
GTATTAG12000.014.9541661
TAACGAA5300.014.660377513
Produced by FastQC (version 0.11.5)