FastQCFastQC Report
Thu 28 Apr 2016
ERR1041943.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041943.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4551784
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC225820.4961131723297942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219360.4819209347367977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204600.4494940884716849No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG175710.3860244686478972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141910.31176786947711055No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA139220.3058580987147017No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG98620.21666230207760295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87200.1915732380974141No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG74870.16448495798570406No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC72790.15991532111365567No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT68260.14996317927212713No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG64500.1417026818495781No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA56480.12408321660254529No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG53540.11762421063916918No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52600.1155590862835319No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC49940.10971522374523923No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT45590.10015853124840722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA5600.017.83928716
AAGACGG12550.017.8366535
ACGGACC12300.017.4471558
GACGGAC11850.017.1729957
CGCAAGA13100.016.6641222
ACGAACG6300.016.15079515
TAACGAA6250.015.98413
GTATTAG14750.015.80339051
ATAACGA6400.015.60937612
CGGACCA13650.015.586089
AACGAAC6450.015.48837214
AGACGGA13600.015.2352946
CAAGACG15250.015.1639354
GCGCAAG14050.015.1423491
CGAGCCG17500.015.11714315
GTTCAAA21050.015.116391
GTACTAG2750.014.7999991
TAAACGC9850.014.64974728
TAACCGG1651.6489139E-814.57575722
ATTAGAG10800.014.2175923
Produced by FastQC (version 0.11.5)