FastQCFastQC Report
Thu 28 Apr 2016
ERR1041942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5617278
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC71060.12650255159171397No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG70220.12500716539220597No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA67890.1208592489102373No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC62700.11161989846327706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA8050.016.3167730
ACGGACC13550.015.9741698
TCTAGCG10050.015.83084528
AAGACGG15500.015.3967735
GTTCAAA20300.015.3103451
GACGGAC14400.015.1597217
TTAACGG4400.015.13636435
ATTAGAG9900.014.9494953
CTAGCGG11100.014.33333329
CTAGTAC2600.014.2307693
CGCAAGA16400.014.2134152
AGACGGA15900.014.1949696
TAATACT9200.014.0760874
TAACGGC4600.014.07608736
TTAGAGT7450.013.906044
TATTAGA8000.013.8750012
CAAGACG17500.013.7428574
CGTTTAT9800.013.59183831
ATACCGC17300.013.58092427
CGGACCA16850.013.5044519
Produced by FastQC (version 0.11.5)