FastQCFastQC Report
Thu 28 Apr 2016
ERR1041941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874783
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA48630.12550380240648315No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC43920.11334828298771828No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT42100.10865124576008516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAAA14500.017.7344841
AAGACGG10050.017.4875625
GTACTAG1301.3960744E-817.0769231
ACGGACC9500.016.3578958
TAGAGTG5150.016.1650495
AATCGTA700.002593982315.85714237
TTAGAGT4450.015.7977534
CGAGCCG14050.015.53736715
GACGGAC10350.015.3719817
TCTATAC2650.015.3584913
ATACCGC13300.015.30075227
GCGAAAG10050.015.27860718
CGCAAGA10500.015.152382
TCGTTTA6600.015.13636430
CAAGACG11650.015.0858374
ATTAGAG6650.015.0225563
TAACGAA5300.015.00943413
TTAACGG3250.014.835
CGAAAGC10500.014.79999919
CTAGCGG7550.014.70198729
Produced by FastQC (version 0.11.5)