FastQCFastQC Report
Thu 28 Apr 2016
ERR1041940.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041940.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758606
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA30420.11027308720418937No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28440.10309554898379834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC1052.2628228E-819.3809533
ATTAGAG4900.018.877553
TCGTTTA4300.018.530
ACGGACC6500.018.4999988
GTATTAA1850.018.01
CGTTTAT4550.017.48351731
GTATAGG3200.017.343751
TATTAGA4500.017.2666662
GACGGAC7250.016.3310347
CGAACGA4450.015.79775316
GTTCAAA8700.015.7356331
AAGACGG7900.015.6898735
AGACGTA1557.221388E-915.5161295
GTATAGA3350.015.4626861
TTAGACT2055.456968E-1215.3414644
GTATTAG8600.015.2732551
CGCAAGA7350.015.102042
TCGAACG2700.015.0740753
ATAACGA4450.014.96629312
TAACGCC5200.014.9423074
Produced by FastQC (version 0.11.5)