FastQCFastQC Report
Thu 28 Apr 2016
ERR1041939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10507365
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA16200.017.70061730
TTAACGG9800.017.17857235
ACGGACC21450.016.904438
TAACGGC10150.016.58620636
CGTTTAT17550.016.44444531
TCTAGCG16100.016.3167728
GACGGAC22150.016.1196387
ATTAGAG20850.016.0599523
CTAGCGG16600.016.04819329
AAGACGG25800.015.3449625
GTATTAG36550.014.8303691
AGACGGA25400.014.6397646
TATACTG13050.014.6015325
TATTAGA20400.014.5098042
TAACGCC21100.014.2037924
GTTCAAA36450.014.1604931
TAAACGC17800.014.1348328
CGGACCA26550.014.0753299
CGCAAGA27150.013.9686922
CGCAATA19350.013.95865636
Produced by FastQC (version 0.11.5)