FastQCFastQC Report
Thu 28 Apr 2016
ERR1041938.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041938.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6607266
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80350.12160854429048264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72910.11034821361815916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70600.10685206256263936No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG67940.10282619165022265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC14800.016.58
GACGGAC15000.016.4033327
TCTAGCG11900.016.3235328
TATTAGA10950.016.0502282
AAGACGG15800.016.041145
TTAACGG6250.015.98435
TAACGGC6650.015.30075236
CTAGCGG13100.015.11068729
CTACGCT1752.244633E-914.84
CGAACGA8950.014.46927316
CGCAAGA18200.014.4340672
GTATTAG21300.014.2441331
AGACGGA17250.014.1565216
TAATACT12850.014.1089494
ATTAGAC4600.014.0760873
CGGACCA18400.014.0760879
TCGTTTA12050.013.97095430
TTAGACT5350.013.8317764
GTTCAAA24800.013.8004031
TAACGAA9450.013.70370413
Produced by FastQC (version 0.11.5)