FastQCFastQC Report
Thu 28 Apr 2016
ERR1041937.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041937.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234693
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT194240.12749846682174693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT171500.11257200916355847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG15450.018.91909235
TAACGGC15550.018.79742836
ATTAGAG36000.016.238893
AAGACGG36050.016.1650495
TCTAGCG25000.015.835999528
GTATTAG52550.015.77164651
ACGGACC34350.015.6186318
TCGTTTA24600.015.4918730
GACGGAC34400.015.4883727
CTAGCGG25750.015.44660229
CGAACGA14950.014.60200616
TATTAGA31500.014.2126982
AGACGGA38350.014.0378096
CGCAAGA39350.013.9631522
TAACGCC28650.013.7539264
TAAACGC28350.013.63844828
ATAACGC34650.013.5613273
CGCAATA30450.013.48768536
CGGACCA40450.013.446239
TCGAACG13250.013.4037733
Produced by FastQC (version 0.11.5)