FastQCFastQC Report
Thu 28 Apr 2016
ERR1041936.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041936.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431893
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC193960.7975679851046078No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG181590.7467022603379342No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA131660.5413889509119028No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG108610.4466068202836227No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT76370.314035198094653No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG73880.30379626077298627No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG70610.2903499454951349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66890.27505321985794606No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC59970.24659802055435825No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA53700.2208156362142578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52330.2151821646758307No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49160.20214705170005423No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC47660.1959790171689297No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT41760.17171808134650662No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG39150.16098570126234993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37520.15428310373852797No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC37220.15304949683230307No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC35450.14577121608557614No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG33920.13947982086382912No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA32570.13392858978581706No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC31080.12780167548490004No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC28550.11739825724240334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC26590.10933869212173399No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT26090.10728268061135912No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC25970.10678923784886918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG3150.018.7936535
TGCGTCG609.240856E-418.510
TACGTTC500.007037085518.49999824
TAACGGC3300.017.93939636
GTACTAG1502.5102054E-1017.2666661
ATTAGAC1452.9849616E-916.5862083
ACGGACC4800.016.18758
GTACAAG3950.015.924051
TTAGACT1659.749783E-1015.6969714
TCGAACG1302.5922418E-715.6538463
TACGTAG1302.5922418E-715.65384614
GTTTAAC4150.015.60240933
ATTAGAG6000.015.4166673
TAGACTA1802.0190782E-1015.4166665
AATACTG4700.015.3510655
GTACTAT4100.015.3414631
AAGACGG5550.015.3333335
TATTAGA6400.015.3203132
GACGGAC5100.015.2352947
AAAGTAC5400.015.0740753
Produced by FastQC (version 0.11.5)