FastQCFastQC Report
Thu 28 Apr 2016
ERR1041935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11362448
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT119190.10489816983100825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG30300.016.9735985
TTAACGG12400.016.85887135
GACGGAC29250.016.4444457
ACGGACC29250.016.3811978
GTATTAG41050.016.314251
TAACGGC12600.016.29761936
ATTAGAG25600.015.7539063
TCTAGCG18000.015.72528
AGACGGA32250.015.6031016
CGCAAGA32200.015.5124232
TCGTTTA18150.015.28925630
CTAGCGG18400.015.08152229
TAAACGC20450.014.74572128
TATTAGA24500.014.3469392
CGGACCA33450.014.3243659
CGAACGA14900.014.15436216
CGAGCCG36500.013.93835715
CAAGACG39700.013.7934514
TATACTG14900.013.7818785
CTAGTAC5650.013.7522133
Produced by FastQC (version 0.11.5)