FastQCFastQC Report
Thu 28 Apr 2016
ERR1041932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699496
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37160.10044611482212713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC6400.017.9218758
TAAACGC5800.016.90517228
TTAGACT3150.016.4444454
GTAAACG6000.016.34166727
TAACGCC5950.016.1680664
TTTAGCG1951.8189894E-1216.12820626
TCGTTTA5450.015.95412930
GACGGAC7400.015.757
GGTATCA28250.015.7168141
TTAACGG2400.015.41666635
TATCCCG1455.3556505E-815.3103455
AAGACGG7850.015.0828035
TTATACC5050.015.0198014
CTAGTAC1853.0559022E-1015.03
TCGAACG3600.014.9027783
CGAACGT3550.014.5915494
GTATTAG10950.014.191781
CGAGCCG10450.014.1626815
AATAACG6150.014.1382112
CTAAGCG1051.6571983E-414.0952399
Produced by FastQC (version 0.11.5)