FastQCFastQC Report
Thu 28 Apr 2016
ERR1041931.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041931.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3598804
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102120.28376093835618726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93380.2594750922806577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64470.17914284856858No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT44080.12248513672875766No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA39440.10959196444152002No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT36140.10042225139240703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA500.00703772418.514
TCGTTTA6600.018.21969630
ACGGACC10500.017.4428588
TCTAGCG7050.017.05673828
GACGGAC10750.016.5209317
AAGACGG12050.016.4273855
CGCAAGA11750.016.3744682
CTAGCGG7400.016.2529
TATACCG1951.8189894E-1216.1282065
ACGAACG6200.015.81451515
CGGACCA12050.015.3526979
CGCATCG8250.015.24848613
GTACCGT855.368096E-415.2352946
ATACCGA2201.8189894E-1215.1363646
CGAACGA6250.015.09616
TAACGCC9200.014.8804354
CCGATAA6350.014.8582689
TAACGAA6350.014.85826813
ATTAGAG6900.014.7463763
GCGCAAG13000.014.6576931
Produced by FastQC (version 0.11.5)