FastQCFastQC Report
Thu 28 Apr 2016
ERR1041929.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041929.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6457064
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96330.1491854502293922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84920.13151488044721255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73300.11351908545431794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.018.3084011
TCTATAC9900.015.1363643
TCTAGCG5950.014.61344528
ACGGACC9050.013.9005538
GTATTAG19200.013.8749991
ATTAGAG10250.013.5365853
TACGTAG5200.013.51923114
CTACCCG6050.013.45454510
TTAGACT6950.013.3093534
GACGGAC9450.012.9206357
CTAGCGG6600.012.89393929
TAACGCC6750.012.6074084
CGAACGT2805.456968E-1212.5535724
CGCAATA8000.012.487536
TAATACT18100.012.4696134
CTCTATA7300.012.4178092
CGGATCG3300.012.33333337
GTACGTA5000.012.21000113
GTATTAA8200.012.1829261
AAGACGG10500.012.1571445
Produced by FastQC (version 0.11.5)