FastQCFastQC Report
Thu 28 Apr 2016
ERR1041928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6626461
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143120.2159825584124014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134180.2024911940174401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102830.15518087256531052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG9950.016.17587929
TCTAGCG10200.016.14215728
GGTATCA69650.016.0961951
GACGGAC14100.016.0070927
ACGGACC14150.015.8197888
AAGACGG15800.014.8702535
CGCAATA11600.014.83189736
TTAGACT5800.014.0344834
TATACTG9900.014.0151525
TCGTTTA8850.013.7966130
TAAACGC10600.013.78773628
GTATTAG17050.013.6715551
CGAACGA7050.013.645390516
AGACGGA16950.013.4247796
CGCAAGA16950.013.4247792
GTAAACG10900.013.40825727
CTAGTAC3450.013.4057973
TAACGCC10100.013.3712864
CGGTCCA13400.013.25373210
TAGCGGC12550.013.11952230
Produced by FastQC (version 0.11.5)