FastQCFastQC Report
Thu 28 Apr 2016
ERR1041927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871040
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131580.2241170218564343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118170.2012760941843353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83560.14232572082629313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAT1403.481E-815.8571431
GACGGAC11300.015.389387
TATACTG8850.015.2598875
AAGACGG12500.015.0965
ACGGACC12100.014.9834718
GTATACG2450.014.3469381
GTATTAG14350.014.1811861
TCTAGCG6900.013.94202828
CGAACGA5200.013.87516
CAAGACG14600.013.8116444
CGGACCA13550.013.5166059
TACGACG9350.013.4545455
TAATACG2950.013.1694924
GGTATCA80800.013.1652231
CGCAAGA15100.012.9867552
CGACGGT9300.012.7311827
CTAGCGG8000.012.71875129
TCTATAC7150.012.6783213
TAACGCC7650.012.5751634
TACGGGT3100.012.5322584
Produced by FastQC (version 0.11.5)