FastQCFastQC Report
Thu 28 Apr 2016
ERR1041926.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041926.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885395
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT128420.6811304792894857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102670.5445543241601892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70390.373343516875774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38840.20600457729016997No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC36150.1917370100164687No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30150.15991343988925397No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA27820.14755528682318558No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT27020.14331214413955698No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26030.13806125506856654No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA22300.11827760230614805No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA22100.1172168166352409No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC21640.11477700959215444No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG21010.11143553472879689No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20260.10745758846289505No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG19880.10544209568817144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1409.458745E-1118.51
CAAGACG7050.017.8439714
TCGTTTA4150.017.83132630
AAGACGG7100.017.4577475
ATACCGG650.001580441317.0769236
ACGGACC7050.016.7943278
GACGGAC7050.016.7943277
GTATAGA1554.0199666E-1016.7096771
GCGCAAG8050.016.5465851
GTATACG1251.6592458E-716.2799991
CGAACGA4150.016.04819316
CGCAAGA7950.015.8238982
TAGGACA1659.749783E-1015.696974
TATTCCG957.063511E-515.5789485
GCAAGAC8100.015.5308643
CGAGCCG6600.015.41666715
ACGAACG4450.015.38202315
TTATACT2650.015.3584914
CTTAGCG855.366413E-415.2352943
AGACGGA7900.015.22151856
Produced by FastQC (version 0.11.5)